Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ETHE1 antibody at 1:500 dilution.Application
ETHE1 antibody detects ETHE1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ETHE1 protein stained by ETHE1 antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:500. Blue: Hoechst 33342 staining.Application
ETHE1 antibody detects ETHE1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: ETHE1 protein stained by ETHE1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE diluted at 1:1000| Product Name | ETHE1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ETHE1 (ethylmalonic encephalopathy 1)This gene encodes a protein that is expressed in the thyroid. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ETHE1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (91%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HSCO antibody YF13H12 antibody ETHE1 antibody protein ETHE1 mitochondrial antibody ethylmalonic encephalopathy protein 1 antibody hepatoma subtracted clone one protein antibody ethylmalonic encephalopathy 1 antibody |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Nucleus , Mitochondrion matrix |
Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ETHE1 antibody at 1:500 dilution.
Application
ETHE1 antibody detects ETHE1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ETHE1 protein stained by ETHE1 antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
ETHE1 antibody detects ETHE1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: ETHE1 protein stained by ETHE1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE diluted at 1:1000| Positive Control | Mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
