Application
Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:5000Application
ETHE1 antibody detects ETHE1 protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract 15 % SDS-PAGE ETHE1 antibody dilution: 1:500Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ETHE1 antibody at 1:500 dilution.Application
ETHE1 antibody detects ETHE1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: ETHE1 protein stained by ETHE1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | ETHE1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ETHE1 (ethylmalonic encephalopathy 1)This gene encodes a protein that is expressed in the thyroid. [provided by RefSeq] |
| Immunogen | Full length human ETHE1 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (90%), Rat (88%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HSCO antibody YF13H12 antibody ETHE1 antibody protein ETHE1 mitochondrial antibody ethylmalonic encephalopathy protein 1 antibody hepatoma subtracted clone one protein antibody ethylmalonic encephalopathy 1 antibody |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus , Mitochondrion matrix |
Application
Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:5000
Application
ETHE1 antibody detects ETHE1 protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract 15 % SDS-PAGE ETHE1 antibody dilution: 1:500
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ETHE1 antibody at 1:500 dilution.
Application
ETHE1 antibody detects ETHE1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: ETHE1 protein stained by ETHE1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | HepG2 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
