Application
Western blot analysis of extracts from 293 cells using PRDM3 antibody .| Product Name | EVI1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [47AT612.84.88] to PRDM3Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. MDS 1, first identified as part of a fusion transcript resulting from the t(3;21)(q26;q22), encodes a small protein of unknown function. EVI 1 encodes a zinc finger protein inappropriately overexpressed by chromosomal rearrangements. EVI 1 and MDS 1 are unusual in that they can either encode separate proteins, or they can be expressed as a fusion protein MDS 1 / EVI 1. EVI 1 and MDS 1 / EVI 1 have opposite functions as transcription factors. |
| Immunogen | Purified recombinant fusion protein encoding the N-terminal part of human PRDM 3. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 47AT612.84.88 |
| Host Species | Mouse |
| Tested Applications | WB |
| ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/100 - 1/500. Predicted molecular weight: 130 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | AML1-EVI-1 EVI1 MDS1 MDS1-EVI1 MGC163392 MGC97004 PRDM3 MDS1 EVI1 AML1 EVI 1 MDS1EVI1 AML1EVI1 |
|---|---|
| Target | PRDM3 |
Application
Western blot analysis of extracts from 293 cells using PRDM3 antibody .| Positive Control | 293 cell lysate |
|---|---|
| Application Notes | ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/100 - 1/500. Predicted molecular weight: 130 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.1% sodium azide |
