Application
Western blot analysis of BF Antibody (Center) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). BF (arrow) was detected using the purified Pab.Application
BF Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Factor B antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Factor B (Internal)This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 401~431 amino acids from the Center region of human BF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | C3/C5 convertase Complement factor B Complement factor B Bb fragment CFB GBG Glycine-rich beta glycoprotein BFD Properdin factor B BF Complement factor B Ba fragment Glycinerich beta glycoprotein PBF2 Glycine rich beta glycoprotein |
|---|---|
| Target | BF |
| Molecular Weight(MW) | 85.533 kDa (note) |
Application
Western blot analysis of BF Antibody (Center) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). BF (arrow) was detected using the purified Pab.
Application
BF Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
