Application
ELISA analysis of Factor VIII recombinant protein using Factor VIII antibody [RFF-VIIIC/8].| Product Name | Factor VIII antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [RFF-VIIIC/8] to Factor VIIIThis gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008] |
| Immunogen | Affinity purified human Factor VIII |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | RFF-VIIIC/8 |
| Host Species | Mouse |
| Tested Applications | ELISARIAWB |
| ELISA: 1/500 - 1/15000 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | HumanPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FVIII F8 HEMA F8C coagulation factor VIII procoagulant component AHF DXS1253E F8B |
|---|---|
| Target | Factor VIII |
| Tissue Specificity | This antibody recognizes the 210kDa, 90kDa and 40kDa cleavage products. Clone RFF-VIIIC/8 does not cross-react with von Willebrand factor. |
Application
ELISA analysis of Factor VIII recombinant protein using Factor VIII antibody [RFF-VIIIC/8].| Application Notes | ELISA: 1/500 - 1/15000 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide (NaN3) |
