Application
Immunofluorescence of FAM59A in Human Liver cells with FAM59A antibody at 20 ug/mL.Application
Western blot analysis of FAM59A in rat liver tissue lysate with FAM59A antibody at (A) 1 and (B) 2 ug/ml.Application
Immunohistochemistry of PLEKHM3 in human brain tissue with PLEKHM3 antibody at 5 ug/ml.| Product Name | FAM59A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to FAM59AFAM59A, also known as GAREM, was initially identified through a mass spectroscoptic method that measures different phosphotyrosine states of proteins in response to different cell stimuli. FAM59A is a downstream molecule in the EGF signaling pathway that is tyrosine-phosphorylated, and this phosphorylation is needed for the binding of Grb2, an adaptor protein crucial to the transduction of growth signals from the plasma membrane to the nucleus. ERK activation in response to EGF stimulation is regulated by FAM59A in COS-7 and HeLa cells, and the overexpression of FAM59A stimulated cell proliferation and colony formation in soft agar, suggesting that FAM59A might be a critical protein with roles in ligand-mediated signaling pathway of the EGF receptor and the tumorigenesis of cells. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Gm944 FAM59A Grb2-associated and regulator of ERK/MAPK Grb2associated and regulator of ERK/MAPK GAREM C18orf11 |
|---|---|
| Target | FAM59A |
| Tissue Specificity | This antibody is specific for FAM59A |
Application
Immunofluorescence of FAM59A in Human Liver cells with FAM59A antibody at 20 ug/mL.
Application
Western blot analysis of FAM59A in rat liver tissue lysate with FAM59A antibody at (A) 1 and (B) 2 ug/ml.
Application
Immunohistochemistry of PLEKHM3 in human brain tissue with PLEKHM3 antibody at 5 ug/ml.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
