Application
ApplicationApplication
Immunohistochemistry: FANCD2 Antibody - Staining of human prostate, glandular epitheliumApplication
FancD2 colocalizes in vivo with another protein in U2OS cells after cell exposure to IR. Proliferating U2OS cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h. Images were captured in a Kodak digital image system on a Leica fluorescence microscope. Working dilution of FancD2 polyclonal antibody is 1:500Application
FancD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. Proliferating SiHa cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h. Images were captured in a Kodak digital image system on a Leica fluorescence microscope. Working dilution of FancD2 polyclonal antibody is 1:500Application
Western Blot: FANCD2 Antibody - Analysis of FANCD2 in human PD20 cells.Application
Detection of human FANCD2 in HeLa whole cell lysate before and after exposure to gamma irradiation using the rabbit polyclonal antibody| Product Name | FANCD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to human Fanconi anemia D2 ( FANCD2 )Fanconi anemia (FANC) is a human autosomal-recessive cancer susceptibility disorder characterized by congenital defects, progressive bone marrow failure, and cellular hypersensitivity to mitomycin C (MMC). The FANC subunit D2 protein is vital for cellular resistance to DNA cross-linking and the arrest of DNA synthesis after ionizing radiation. DNA damage activates the monoubiquitination of FANCD2, targeting nuclear foci containing the BRCA1 protein. |
| Immunogen | Human FANCD2 fusion protein. N-terminal fragment). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB: recognize a band at ~166 kDa (post-translationally modified form). Additional bands may be seen at lower molecular weights. Suggested working dilutions: WB: 1:10,000-1:20,000 (ECL). Immunofluorescence - 1:200-1:500 Immunoprecipitation - (4ul for 1mg whole cell extract) The investigator should determine the optimal working dilution for a specific application. Other applications have not been tested.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FACD FA-D2 2177 FANCD2 FA4 FANCD DKFZp762A223 227646 Q9BXW9 FAD FAD2 FA D2 |
|---|---|
| Target | FANCD2 |
| Tissue Specificity | This antibody is specific for human FANCD2. |
Application
Application
Application
Immunohistochemistry: FANCD2 Antibody - Staining of human prostate, glandular epithelium
Application
FancD2 colocalizes in vivo with another protein in U2OS cells after cell exposure to IR. Proliferating U2OS cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h. Images were captured in a Kodak digital image system on a Leica fluorescence microscope. Working dilution of FancD2 polyclonal antibody is 1:500
Application
FancD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. Proliferating SiHa cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h. Images were captured in a Kodak digital image system on a Leica fluorescence microscope. Working dilution of FancD2 polyclonal antibody is 1:500
Application
Western Blot: FANCD2 Antibody - Analysis of FANCD2 in human PD20 cells.
Application
Detection of human FANCD2 in HeLa whole cell lysate before and after exposure to gamma irradiation using the rabbit polyclonal antibody| Positive Control | HeLa whole cell extracts |
|---|---|
| Application Notes | WB: recognize a band at ~166 kDa (post-translationally modified form). Additional bands may be seen at lower molecular weights. Suggested working dilutions: WB: 1:10,000-1:20,000 (ECL). Immunofluorescence - 1:200-1:500 Immunoprecipitation - (4ul for 1mg whole cell extract) The investigator should determine the optimal working dilution for a specific application. Other applications have not been tested.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 1% BSA and 0.05% sodium azide |
