Application
Western blot analysis of extracts from HT29 cells treated with CalyculinA using Phospho-FANCD2 (Ser222) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | FANCD2 (phospho Ser222) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.1) Ho GP, Margossian S, Taniguchi T, D'Andrea AD (2006)Mol Cell Biol 26, 7005-15. Nakanishi K, et al. (2002)Nat Cell Biol 4, 913-20. Taniguchi T, et al. (2002) Cell 109, 459-72 . |
| Immunogen | FANCD2 (phospho Ser222) antibody was raised against a peptide sequence around phosphorylation site of serine 222 (G-D-S (p)-Q-H) derived from Human FANCD2. |
| Modification | p-Ser222 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FANCD2 |
|---|---|
| Alternative Names | DKFZp762A223 FA-D2 FA4 FACD Fanconi anemia |
| Molecular Weight(MW) | 166 kDa |
| Tissue Specificity | The antibody detects endogenous level of FANCD2 only when phosphorylated at serine 222. |
| Entrez Gene | 2177 |
|---|---|
| Protein Accession | NP_001018125.1 |
Application
Western blot analysis of extracts from HT29 cells treated with CalyculinA using Phospho-FANCD2 (Ser222) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
