Application
Western Blot: FANCJ Antibody [ - Analysis on MCF7, HeLa, 293T and SKOV3 crude cell lysates.Application
Western Blot: FANCJ Antibody [ - Brip 1 detected in cell lysates using Lane 1: MCF-7 lysate, Lane 2: HeLa lysate, Lane 3: 293 lysate and Lane 4: SKOV3 lysateApplication
Immunohistochemistry: FANCJ Antibody [ - Analysis of FANCJ in human colon and rectum tissues| Product Name | FANCJ antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Brip1 / FANCJ (Similar to BRCA1-binding helicase-like protein BACH1)BRIP1 (also called BACH1) is a helicase that interacts with the BRCT domain of BRCA1 and has a role in BRCA1-dependent DNA repair and checkpoint. BRIP1 has recently been found to be defective in Fanconi anemia complementation group J. BRIP1/FANCJ has a function in the Fanconi anemia pathway that is independent of BRCA1 and downstream of FANCD2 activation. (1-4) |
| Immunogen | A synthetic peptide within the extreme C-terminus of the human BACH1/Brip1 protein sequence. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| By Western blot, immunoprecipitation, and chromatin immunoprecipitation, this antibody recognizes a band at ~130 kDa, representing BRIP1. Suggested working dilutions: *Western Blot - 1:2,000-1:3,000 (ECL)Immunoprecipitation - 2-4 ul per IPImmunofluorescence - NDImmunohistochemistry - ND*The investigator should determine the optimal working dilution for a specific application.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FANCJ 114480 BACH1 83990 MGC126521 FLJ90232 BRIP1 OF Q9BX63 MGC126523 |
|---|---|
| Target | BRIP1 |
| Tissue Specificity | This antibody is specific for BACH1/Brip1. |
Application
Western Blot: FANCJ Antibody [ - Analysis on MCF7, HeLa, 293T and SKOV3 crude cell lysates.
Application
Western Blot: FANCJ Antibody [ - Brip 1 detected in cell lysates using Lane 1: MCF-7 lysate, Lane 2: HeLa lysate, Lane 3: 293 lysate and Lane 4: SKOV3 lysate
Application
Immunohistochemistry: FANCJ Antibody [ - Analysis of FANCJ in human colon and rectum tissues| Positive Control | HeLa whole cell extract, and Raji, 293, MCF7 and SKOV3 whole cell extracts |
|---|---|
| Application Notes | By Western blot, immunoprecipitation, and chromatin immunoprecipitation, this antibody recognizes a band at ~130 kDa, representing BRIP1. Suggested working dilutions: *Western Blot - 1:2,000-1:3,000 (ECL)Immunoprecipitation - 2-4 ul per IPImmunofluorescence - NDImmunohistochemistry - ND*The investigator should determine the optimal working dilution for a specific application.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4 degrees Celcius. For long-term storage, store at -20 degrees Celcius. |
| Storage Buffer | 0.1 ml of whole rabbit antisera with 0.01% Sodium Azide. |
