Application
Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE diluted at 1:1000| Product Name | Fas antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EP1 (Fas (TNF receptor superfamily, member 6))The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human EP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ALPS1A antibody APO-1 antibody APT1 antibody CD95 antibody FAS1 antibody FASTM antibody TNFRSF6 antibody FAS antibody tumor necrosis factor receptor superfamily member 6 antibody FAS 827dupA antibody apoptosis-mediating surface antigen FAS antibo |
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| Molecular Weight(MW) | 38 kDa (note) (2) |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted , Isoform 3: Secreted , Isoform 4: Secreted , Isoform 5: Secreted , Isoform 6: Secreted |
Application
Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE diluted at 1:1000| Positive Control | K562 , THP-1 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
