Rabbit Polyclonal antibody to FBXO4 (F-box protein 4)This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the center region of human FBXO4. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ICC/IFWB
*Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
DKFZp547N213 antibody FBX4 antibody FLJ10141 antibody FBXO4 antibody F-box protein Fbx4 antibody F-box only protein 4 antibody F-box protein 4 antibody
Molecular Weight(MW)
44 kDa (note) (3)
Application
Application
Sample (30 ug of whole cell lysate) A: HL-60 10% SDS PAGE diluted at 1:5000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using FBXO4 antibody at 1:500 dilution.
Positive Control
HL-60
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.