Application
Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE diluted at 1:2000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using FBXO4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | FBXO4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FBXO4 (F-box protein 4)This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FBXO4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp547N213 antibody FBX4 antibody FLJ10141 antibody FBXO4 antibody F-box protein Fbx4 antibody F-box only protein 4 antibody F-box protein 4 antibody |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE diluted at 1:2000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using FBXO4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | A431 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
