Application
WB analysis of Jurkat lysate (40ug) using Fem-2 antibody [AT6D11] at a dilution of 1:1,000.| Product Name | Fem-2 antibody [AT6D11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT6D11] to Fem-2The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant human Fem-2 (1-454aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AT6D11 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | protein phosphatase, Mg2+/Mn2+ dependent, 1F , FEM-2 , POPX2 , hFEM-2 , CaMKPase , CAMKP |
|---|---|
| Target | PPM1F |
| Light Chain Type | kappa |
Application
WB analysis of Jurkat lysate (40ug) using Fem-2 antibody [AT6D11] at a dilution of 1:1,000.| Application Notes | The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol, 0.02% Sodium Azide. |
