Application
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody .Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)Application
Immunoprecipitation (IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.| Product Name | FERMT2 antibody [25A4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [25A4] to FERMT2Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. |
| Immunogen | Protein expressed in 293T cell transfected with human FERMT2 expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 25A4 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PIPWB |
| Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FERMT2, KIND2, mig-2, mig2, UNC112, mig 2 |
|---|---|
| Target | FERMT2 |
| Tissue Specificity | is specific for human FERMT2. |
| Cellular Localization | Within actin stress fibers at cell-ECM focal adhesion sites. |
Application
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody .
Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunoprecipitation (IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.| Application Notes | Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
