Application
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody .Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-FERMT2 mouse monoclonal antibody. ( Dilution 1:50)| Product Name | FERMT2 antibody [9E4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [9E4] to FERMT2Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. |
| Immunogen | Protein expressed in 293T cell transfected with human FERMT2 expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 9E4 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanDogMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FERMT2, KIND2, mig-2, mig2, UNC112, mig 2 |
|---|---|
| Target | FERMT2 |
| Tissue Specificity | is specific for human FERMT2. |
| Cellular Localization | Within actin stress fibers at cell-ECM focal adhesion sites. |
Application
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody .
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-FERMT2mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-FERMT2 mouse monoclonal antibody. ( Dilution 1:50)| Application Notes | Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
