Application
Anti-AFGF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with AFGFApplication
description-4Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFGF.| Product Name | FGF1 antibody [4D2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4D2] to FGF1The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 16-155 of human aFGF |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4D2 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ECGFA, HBGF1, ECGF-beta, ECGF, AFGF, ECGFB, FGF1, GLIO703, ECGFbeta, FGFA, FGFalpha, FGF-alpha, ECGF beta, FGF alpha |
|---|---|
| Target | aFGF |
| Tissue Specificity | is specific for amino acids 16-155 of human aFGF |
Application
Anti-AFGF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with AFGF
Application
description-4
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFGF.| Application Notes | Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
