Application
HEK293T cells transfected with either BFGF overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BFGF antibody , and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFGF.| Product Name | FGF2 antibody [3D10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3D10] to BFGFThe heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. |
| Immunogen | Recombinant fragment expressed in E.coli corresponding to amino acids 10--155 of human bFGF |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3D10 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HBGH-2, BFGF, FGF2, HBGH2, FGFB, HBGH 2 |
|---|---|
| Target | BFGF |
| Tissue Specificity | is specific for amino acids 10-155 of human bFGF |
Application
HEK293T cells transfected with either BFGF overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BFGF antibody , and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFGF.| Application Notes | Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
