| Product Name | FGF2 antibody [AS24] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [AS24] to FGF basicFGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AS24 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-P |
| ELISA: This antibody can be used as a coating antibody at 10 μg/ml in a two site ELISA with biotin conjugated bFGF antibody. Typically the ELISA has a working range of 0-2000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 μl. Recovery of human basic FGF in human serum is typically > 85% in the ELISA. IHC: This antibody can be used at 1-10 ug/ml to stain human basic FGF expressed in mononuclear cells, following fixation with 4% paraform in PBS for 10minutes at 48C, plus 0.1% saponin for 20minutes at room temperature. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | HBGH-2, FGF basic, P09038, 2247, 134920, BFGF, FGF2, HBGH2, FGFB, HBGF-2, HBGH 2, HBGF 2, HBGF2 |
|---|---|
| Target | FGF basic |
| Tissue Specificity | Recognizes human basic FGF. It does not recognize human acidic FGF or a panel of other human cytokines. |
| Cellular Localization | Secreted |
| Application Notes | ELISA: This antibody can be used as a coating antibody at 10 μg/ml in a two site ELISA with biotin conjugated bFGF antibody. Typically the ELISA has a working range of 0-2000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 μl. Recovery of human basic FGF in human serum is typically > 85% in the ELISA. IHC: This antibody can be used at 1-10 ug/ml to stain human basic FGF expressed in mononuclear cells, following fixation with 4% paraform in PBS for 10minutes at 48C, plus 0.1% saponin for 20minutes at room temperature. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-bufferd saline containing 0.08% sodium azide |
