Application
Western blot analysis of extracts of various cell lines, using FBL antibody.Application
Immunofluorescence analysis of A549 cell using FBL antibody. Blue: DAPI for nuclear staining.Application
Immunofluorescence analysis of HeLa cell using FBL antibody. Blue: DAPI for nuclear staining.| Product Name | Fibrillarin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FibrillarinThis gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human FBL |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPRIPWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:20 - 1:50, RIP 1:20 - 1:50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FBL FIB RNU3IP1 FLRN fibrillarin |
|---|---|
| Target | Fibrillarin |
| Molecular Weight(MW) | 34 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using FBL antibody.
Application
Immunofluorescence analysis of A549 cell using FBL antibody. Blue: DAPI for nuclear staining.
Application
Immunofluorescence analysis of HeLa cell using FBL antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:20 - 1:50, RIP 1:20 - 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
