Application
Western blot analysis of extract from 293, Hela cells using Fibrillarin Antibody| Product Name | Fibrillarin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FibrillarinInvolved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. |
| Immunogen | Peptide sequence around aa.297~301( L-T-L-E-P) derived from Human Fibrillarin |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FIB FLRN RNU3IP1 FBL 34 kDa nucleolar scleroderma antigen RNA U3 small nucleolar interacting protein 1 rRNA 2'-O-methyltransferase fibrillarin 34-kD nucleolar scleroderma antigen fibrillarin |
|---|---|
| Target | Fibrillarin |
| Molecular Weight(MW) | 37 (note) |
Application
Western blot analysis of extract from 293, Hela cells using Fibrillarin Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
