Mouse monoclonal [FLG01] to FilaggrinFilaggrin is expressed only in well differentiated keratinized epithelial cells. The synthesis of filaggrin varies in types of ichthyosis, and with viral, premalignant and malignant conditions.
Immunogen
Recombinant full length protein
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
FLG01
Host Species
Mouse
Tested Applications
ICC/IFIHC-PWB
Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 2 - 4μg / ml for 30 min at RT. Optimal dilutions / concentrations should be determined by the end user. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.:
Species Reactivity
Human
Concentration
0.2mg/ml
Purification
Protein G
Target Information
Gene Synonyms
P20930 Filaggrin 135940 FLG 2312 ATOD2
Target
Filaggrin
Cellular Localization
Cytoplasmic.
Light Chain Type
kappa
Positive Control
Skin
Application Notes
Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 2 - 4μg / ml for 30 min at RT. Optimal dilutions / concentrations should be determined by the end user. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.