Application
Filamin A antibody detects Filamin A protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 5 % SDS-PAGE Filamin A antibody dilution: 1:1000Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 5% SDS PAGE diluted at 1:1000Application
Filamin A antibody [C2], C-term detects Filamin A protein at cytosol on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Filamin A antibody [C2], C-term dilution: 1:500.Application
Sample (50 ug of whole cell lysate) A: mouse kidney 5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using Filamin A alpha 2 antibody at 1:200 dilution.| Product Name | Filamin A antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Filamin A (filamin A, alpha)The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Filamin A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rabbit, BovineRabbit (98%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ABP-280 antibody ABPX antibody CVD1 antibody DKFZp434P031 antibody FLN antibody FLN-A antibody FLN1 antibody FMD antibody MNS antibody NHBP antibody OPD antibody OPD1 antibody OPD2 antibody XLVD antibody XMVD antibody FLNA antibody filamin- |
|---|---|
| Molecular Weight(MW) | 281 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cell cortex , cytoskeleton |
Application
Filamin A antibody detects Filamin A protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 5 % SDS-PAGE Filamin A antibody dilution: 1:1000
Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 5% SDS PAGE diluted at 1:1000
Application
Filamin A antibody [C2], C-term detects Filamin A protein at cytosol on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Filamin A antibody [C2], C-term dilution: 1:500.
Application
Sample (50 ug of whole cell lysate) A: mouse kidney 5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Filamin A alpha 2 antibody at 1:200 dilution.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse kidney , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
