Application
Western Blot: Luciferase Antibody (Luci 21 1-107) - WB analysis of on Luciferase ProteinApplication
Immunohistochemistry: Luciferase Antibody [ - Detection of luciferase expression in CD34+ cells by immunofluorescence. Cytospin slides prepared from transduced CD34+ cells after 3 days of culture were stained with monoclonal antiluciferase antibody. Luciferase-positive cells have green cytoplasm; nuclei stained with DAPI are blue. Nontransduced, cultured CD34+ cells were used as a negative control. Original magnification, x 40. Wang, X. et al., Dynamic tracking of human hematopoietic stem cell. Blood. 102(10): 3478-3482, 2003.| Product Name | Firefly Luciferase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [Luci 21 1 - 107] to firefly LuciferaseLuciferase is a luminescent enzyme, mostly known for its role as a reporter protein. |
| Immunogen | Luciferase protein from firefly, Photinus pyralis |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | Luci 21 1-107 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| For WB: Use at a dilution of 1:1,000 on Drosophila embryos. Purified luciferase protein and luciferase expressed in Drosophila adult co-migrate on Western blots with a band seen at ~61 kDa, representing luciferase. Does not appear to work in immunohistochemistry (paraffin-embedded sections). Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ec 1.13.12.7 Luciferin4 monooxygenase firefly Luciferase ffLuc ec1.13.12.7 Luciferin 4 monooxygenase Luciferase |
|---|---|
| Target | Firefly Luciferase |
| Tissue Specificity | Specific for luciferase, recognizing a peptide consisting of the first 258 amino acids. Further epitope mapping has not been done at this time. |
| Light Chain Type | kappa |
Application
Western Blot: Luciferase Antibody (Luci 21 1-107) - WB analysis of on Luciferase Protein
Application
Immunohistochemistry: Luciferase Antibody [ - Detection of luciferase expression in CD34+ cells by immunofluorescence. Cytospin slides prepared from transduced CD34+ cells after 3 days of culture were stained with monoclonal antiluciferase antibody. Luciferase-positive cells have green cytoplasm; nuclei stained with DAPI are blue. Nontransduced, cultured CD34+ cells were used as a negative control. Original magnification, x 40. Wang, X. et al., Dynamic tracking of human hematopoietic stem cell. Blood. 102(10): 3478-3482, 2003.| Positive Control | Purified Luciferase protein or Luciferase expressed in Drosophila |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:1,000 on Drosophila embryos. Purified luciferase protein and luciferase expressed in Drosophila adult co-migrate on Western blots with a band seen at ~61 kDa, representing luciferase. Does not appear to work in immunohistochemistry (paraffin-embedded sections). Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid containing 0.1% sodium azide |
