Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT4211] diluted at 1:500.Application
FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: FIS1 protein stained by FIS1 antibody diluted at 1:1000. Red: MitoTrackerR Red CMXRos, a mitochondria tracker. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
FIS1 antibody detects FIS1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody diluted by 1:1000.Application
FIS1 antibody [GT4211] detects FIS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: FIS1 protein stained by FIS1 antibody [GT4211] diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | FIS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT4211] to FIS1 (fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae))The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM] |
| Immunogen | Full length human FIS1 Recombinant protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT4211 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (96%), Rat (87%), Rhesus Monkey (100%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CGI-135 fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) TTC11 |
|---|---|
| Target | FIS1 |
| Molecular Weight(MW) | 17 kDa (note) |
| Cellular Localization | Mitochondrion outer membrane; Single-pass membrane protein , Peroxisome membrane; Single-pass membrane protein |
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT4211] diluted at 1:500.
Application
FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: FIS1 protein stained by FIS1 antibody diluted at 1:1000. Red: MitoTrackerR Red CMXRos, a mitochondria tracker. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
FIS1 antibody detects FIS1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody diluted by 1:1000.
Application
FIS1 antibody [GT4211] detects FIS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: FIS1 protein stained by FIS1 antibody [GT4211] diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
