Application
Western blot analysis of extracts of various cell lines, using FLI1 antibody.Application
Immunofluorescence analysis of U20S cell using FLI1 antibody. Blue: DAPI for nuclear staining.| Product Name | FLI1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FLI1This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| Immunogen | Recombinant protein of human FLI1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EWSR2 SIC-1 Fli-1 proto-oncogene ETS transcription factor |
|---|---|
| Target | FLI1 |
| Molecular Weight(MW) | 51 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using FLI1 antibody.
Application
Immunofluorescence analysis of U20S cell using FLI1 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
