Application
Immunocytochemical staining of FLIP in HeLa cells with FLIP antibody at 5μg/ml.Application
Western blot analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2μg/ml.| Product Name | FLIP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FLIPApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively (1,2). The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP (3-7). FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and-10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPa, b, g, and d, respectively (8). |
| Immunogen | Peptide corresponding to amino acids 2 to 18 of human FLIP2. The sequence is identical in all FLIP splice variants. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Western blot at1:500 to 1:1000 dilution.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Gene Synonyms | Q01543 SIC1 SIC-1 FLI1 FLIP EWSR2 2313 193067 CASH CASP8AP1 CLARP Casper FLAME FLAME-1 FLAME1 I-FLICE MRIT c-FLIP c-FLIPL c-FLIPR c-FLIPS SIC 1 c FLIP FLAME 1 I FLICE c FLIPL c FLIPR c FLIPS cFLIP IFLICE cFLIPL cFLIPR cFLIPS |
|---|---|
| Target | FLIP |
| Tissue Specificity | Recognizes all FLIP splice variants including FLIP alpha, beta, gamma, and delta. |
| Cellular Localization | Cytoplasmic |
Application
Immunocytochemical staining of FLIP in HeLa cells with FLIP antibody at 5μg/ml.
Application
Western blot analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2μg/ml.| Application Notes | Western blot at1:500 to 1:1000 dilution.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | PBS with 0.02% sodium azide |
