Application
Immunocytochemical staining of FLIPα in Jurkat cells with FLIPα antibody at 10μg/ml.Application
Western blot analysis of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysate with FLIP antibody at 1ug/ml. Positive control: Whole cell lysate from HeLa cells.| Product Name | FLIP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FLIPApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively1, 2. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and-10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPa, b, g, and d, respectively8. |
| Immunogen | Synthetic peptide, corresponding to amino acids 447-464 of Human FLIP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| For WB: Use at a dilution of 1:500 - 1:2,000. Optimal concentrations/dilutions should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Q01543 SIC1 SIC-1 FLI1 FLIP EWSR2 2313 193067 CASH CASP8AP1 CLARP Casper FLAME FLAME-1 FLAME1 I-FLICE MRIT c-FLIP c-FLIPL c-FLIPR c-FLIPS SIC 1 c FLIP FLAME 1 I FLICE c FLIPL c FLIPR c FLIPS cFLIP IFLICE cFLIPL cFLIPR cFLIPS |
|---|---|
| Target | FLIP |
| Tissue Specificity | This antibody recognizes full-length human FLIP (55 kD). FLIP has a short form (FLIPS) and a long form (FLIPL); this antibody recognizes FLIPL only. |
| Cellular Localization | Cytoplasmic |
Application
Immunocytochemical staining of FLIPα in Jurkat cells with FLIPα antibody at 10μg/ml.
Application
Western blot analysis of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysate with FLIP antibody at 1ug/ml. Positive control: Whole cell lysate from HeLa cells.| Positive Control | HeLa cells |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500 - 1:2,000. Optimal concentrations/dilutions should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.4 with 0.02% sodium azide as preservative. |
