Application
Flotillin 2 antibody detects Flotillin 2 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and blotted with Flotillin 2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 μg of Flotillin 2 antibody [C3], C-term . Western blot analysis was performed using Flotillin 2 antibody [C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using Flotillin-2 antibody at 1:100 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLaS3, using Flotillin-2 antibody at 1:100 dilution.Application
Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis. A. 50 µg mouse brain lysate/extract 10% SDS-PAGE Flotillin 2 antibody [C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis. A. 50 µg rat brain lysate/extract 10% SDS-PAGE Flotillin 2 antibody [C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Flotillin 2 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Flotillin 2 (flotillin 2)Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ECS-1 antibody ECS1 antibody ESA antibody ESA1 antibody M17S1 antibody FLOT2 antibody membrane component chromosome 17 surface marker 1 antibody flotillin-2 antibody epidermal surface antigen antibody membrane component chromosome 17 surface mar |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (2) |
| Cellular Localization | Cell membrane; Peripheral membrane protein , Membrane , caveola; Peripheral membrane protein |
Application
Flotillin 2 antibody detects Flotillin 2 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and blotted with Flotillin 2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 μg of Flotillin 2 antibody [C3], C-term . Western blot analysis was performed using Flotillin 2 antibody [C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using Flotillin-2 antibody at 1:100 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLaS3, using Flotillin-2 antibody at 1:100 dilution.
Application
Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis. A. 50 µg mouse brain lysate/extract 10% SDS-PAGE Flotillin 2 antibody [C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis. A. 50 µg rat brain lysate/extract 10% SDS-PAGE Flotillin 2 antibody [C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
