| Product Name | Fluorescein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2A3] to FluoresceinFITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. |
| Immunogen | FITC-BSA conjugate. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 2A3 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| IHC: Use at a dilution of 1:250. Detection of FITC-labeled antigens or antibodies. Capture antibody for FITC-labeled biopolymers. Enzyme-labeled second antibody for immunohistochemistry with FITC-conjugated mouse monoclonal antibodies. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Other |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | Fluorescein |
|---|---|
| Target | Fluorescein |
| Tissue Specificity | This antibody reacts with both free fluorescein (FITC) and conjugated FITC, so it recognizes FITC-labeled proteins.Kd = 1,5 x 10-8 |
| Application Notes | IHC: Use at a dilution of 1:250. Detection of FITC-labeled antigens or antibodies. Capture antibody for FITC-labeled biopolymers. Enzyme-labeled second antibody for immunohistochemistry with FITC-conjugated mouse monoclonal antibodies. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide |
