Application
Western blot analysis of FRS2 (pY436) expression in Jurkat (A); Huvec (B) whole cell lysates.Application
Immunohistochemical analysis of FRS2 (pY436) staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | FRS2 (phospho Tyr436) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FRS2 (phospho Tyr436) |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human FRS2 (pY436). The exact sequence is proprietary. |
| Modification | p-Tyr436 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | fibroblast growth factor receptor substrate 2 FRS2alpha SNT1 FRS2A FRS2 SNT SNT-1 |
|---|---|
| Target | FRS2 (phospho Tyr436) |
| Tissue Specificity | Recognizes endogenous levels of FRS2 (pY436) protein. |
Application
Western blot analysis of FRS2 (pY436) expression in Jurkat (A); Huvec (B) whole cell lysates.
Application
Immunohistochemical analysis of FRS2 (pY436) staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
