Application
Hela whole cell and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
FUBP1 antibody detects FUBP1 protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. FUBP1 antibody dilution: 1:1000.Application
FUBP1 antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
FUBP1 antibody detects FUBP1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. FUBP1 antibody dilution: 1:1000.Application
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-FUBP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 FUSE.Application
FUBP-1 antibody immunoprecipitates FUBP-1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of FUBP-1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP-1 by 2.5 μg of FUBP-1 antibody 7.5% SDS-PAGE The immunoprecipitated FUBP-1 protein was detected by FUBP-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Product Name | FUBP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FUBP1 (far upstream element-binding protein 1)This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FUBP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Chicken, BovineRat (99%), Zebrafish (83%), Xenopus laevis (91%), Chicken (94%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FBP antibody FUBP antibody FUBP1 antibody hDH V antibody FUSE-binding protein 1 antibody far upstream element binding protein antibody DNA helicase V antibody far upstream element-binding protein 1 antibody far upstream element (FUSE) binding prote |
|---|---|
| Molecular Weight(MW) | 68 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Hela whole cell and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
FUBP1 antibody detects FUBP1 protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. FUBP1 antibody dilution: 1:1000.
Application
FUBP1 antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
FUBP1 antibody detects FUBP1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. FUBP1 antibody dilution: 1:1000.
Application
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-FUBP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 FUSE.
Application
FUBP-1 antibody immunoprecipitates FUBP-1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of FUBP-1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP-1 by 2.5 μg of FUBP-1 antibody 7.5% SDS-PAGE The immunoprecipitated FUBP-1 protein was detected by FUBP-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Positive Control | Jurkat , K562 , THP-1 , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
