Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody diluted at 1:1000.Application
GABARAP antibody detects GABARAP protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 whole cell lysate/extract of human GABARAP -transfected 293T cells 15 % SDS-PAGE GABARAP antibody dilution: 1:10000Application
GABARAP antibody detects GABARAP protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract 15 % SDS-PAGE GABARAP antibody dilution: 1:2000Application
GABARAP antibody detects GABARAP protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 15 % SDS-PAGE GABARAP antibody dilution: 1:2000Application
GABARAP antibody detects GABARAP protein at cytoplasm by immunofluorescent analysis. Sample: U2OS cells were fixed in ice-cold MeOH for 5 min. Green: GABARAP protein stained by GABARAP antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | GABARAP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GABARAP (GABA(A) receptor-associated protein)Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GABARAP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rabbit, Rhesus Monkey, BovineMouse (100%), Rat (100%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GABA(A) receptor-associated protein GABARAP-a ATG8A MM46 |
|---|---|
| Target | GABARAP |
| Molecular Weight(MW) | 14 kDa (note) |
| Cellular Localization | Endomembrane system (By similarity) , Cytoplasm , cytoskeleton (By similarity) , Golgi apparatus membrane (By similarity) |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody diluted at 1:1000.
Application
GABARAP antibody detects GABARAP protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 whole cell lysate/extract of human GABARAP -transfected 293T cells 15 % SDS-PAGE GABARAP antibody dilution: 1:10000
Application
GABARAP antibody detects GABARAP protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract 15 % SDS-PAGE GABARAP antibody dilution: 1:2000
Application
GABARAP antibody detects GABARAP protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 15 % SDS-PAGE GABARAP antibody dilution: 1:2000
Application
GABARAP antibody detects GABARAP protein at cytoplasm by immunofluorescent analysis. Sample: U2OS cells were fixed in ice-cold MeOH for 5 min. Green: GABARAP protein stained by GABARAP antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , Jurkat , Raji , NCI-H929 , A431 , HeLa , human GABARAP-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
