WB
Western blot analysis of GABARAP using anti-GABARAP antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HELA whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: human HEPG2 whole cell lysates, Lane 7: human U937 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: rat pancreas tissue lysates, Lane 10: mouse liver tissue lysates, Lane 11: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GABARAP antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for GABARAP at approximately 18 kDa. The expected band size for GABARAP is at 18 kDa.IHC
IHC analysis of GABARAP using anti-GABARAP antibody . GABARAP was detected in a paraffin-embedded section of human renal carcinomar tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GABARAP Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.FC
Flow Cytometry analysis of Hepa1-6 cells using anti-GABARAP antibody. Overlay histogram showing Hepa1-6 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GABARAP Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-GABARAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human GABARAP, identical to the related mouse and rat sequences. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-2000 FC:1:50-200 IHC:1:50-400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GABARAP |
|---|---|
| Gene Synonyms | MM46 ATG8A GABARAP-a |
| Gene Full Name | GABA type A receptor-associated protein |
| Gene Summary | Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq, Jul 2008] |
| Alternative Names | FLC3B GABARAP MM46 |
| Molecular Weight(MW) | 18 kDa |
| Cellular Localization | Cytoplasm,Cytoplasmic vesicle,Cytoskeleton,Golgi apparatus,Membrane,Microtubule |
WB
Western blot analysis of GABARAP using anti-GABARAP antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HELA whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: human HEPG2 whole cell lysates, Lane 7: human U937 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: rat pancreas tissue lysates, Lane 10: mouse liver tissue lysates, Lane 11: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GABARAP antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for GABARAP at approximately 18 kDa. The expected band size for GABARAP is at 18 kDa.
IHC
IHC analysis of GABARAP using anti-GABARAP antibody . GABARAP was detected in a paraffin-embedded section of human renal carcinomar tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GABARAP Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FC
Flow Cytometry analysis of Hepa1-6 cells using anti-GABARAP antibody. Overlay histogram showing Hepa1-6 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GABARAP Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 FC:1:50-200 IHC:1:50-400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
