Application
Immunofluorescence of GABARAP in human brain tissue with GABARAP antibody at 20 μg/ml.Application
Immunofluorescence of GABARAP in mouse brain tissue with GABARAP Antibodyat 20 μg/mL.Application
Immunohistochemistry of GABARAP in mouse brain tissue with GABARAP Antibodyat 5 μg/mL.Application
Western blot analysis of GABARAP in HeLa cell lysate with GABARAP antibody at 1 μg/ml.| Product Name | GABARAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GABARAP Antibody: MM46, ATG8A, GABARAP-a, FLC3B, HT004, Gamma-aminobutyric acid receptor-associated protein, MM46 |
| Product description | GABARAP Antibody: Gamma-aminobutyric acid (GABA) is the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing in the central nervous system. It has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. GABARAP (GABAA receptor-associated protein) links GABAA receptors to the cytoskeleton and may play a role in intracellular transport of GABAA receptors and its interaction with the cytoskeleton. GABARAP belongs to the MAP1 or ATG8 like family and recent studies show that MAPK15/ERK8 is acting through interaction with ATG8 family proteins to regulate autophagy.1) Cherubini E, Gaiarsa JL, and Ben-Ari Y. GABA: an excitatory transmitter in early postnatal life. Trends Neurosci.1991; 14:515-19. |
| Immunogen | GABARAP antibody was raised against a 19 amino acid peptide near the amino terminus of human GABARAP. The immunogen is located within the first 50 amino acids of GABARAP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC-P |
| GABARAP antibody can be used for detection of GABARAP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GABARAP |
|---|---|
| Alternative Names | GABARAP Antibody: MM46 ATG8A GABARAP-a FLC3B HT004 Gamma-aminobutyric acid receptor-associated protein MM46 |
| Molecular Weight(MW) | Predicted: 13kDa Observed: 15 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | Multiple isoforms of GABARAP exist due to alternative splicing events. |
Application
Immunofluorescence of GABARAP in human brain tissue with GABARAP antibody at 20 μg/ml.
Application
Immunofluorescence of GABARAP in mouse brain tissue with GABARAP Antibodyat 20 μg/mL.
Application
Immunohistochemistry of GABARAP in mouse brain tissue with GABARAP Antibodyat 5 μg/mL.
Application
Western blot analysis of GABARAP in HeLa cell lysate with GABARAP antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | GABARAP antibody can be used for detection of GABARAP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GABARAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | GABARAP Antibody is supplied in PBS containing 0.02% sodium azide. |
