Application
Western Blot: APG8 Antibody [ - immunization with recombinant yeast APG8 was tested with other anti-UBL antibodies against E.coli lysates expressing the APG8-GFP fusion protein. Panel A shows total protein staining using ponceau. Panel B shows specific reaction with APG8 using a 1:4,000 and 1:8,000 dilution of IgG fraction of Rabbit-anti-APG8 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX . All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4C. E.coli lysate proteins were separated by SDS-PAGE using a 15% gel. Similar experiments where other UBL fusion proteins were separated and probed with this antibody showed no reactivity of anti-APG8 with other UBLs.| Product Name | GABARAPL1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to GABARAPL1Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs)function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Apg8 is required for autophagy (intracellular bulk protein degradation) in yeast. Starved yeast cells take up their own cytoplasm into vacuoles through autophagic bodies. Autophagic bodies form a double-membraned structure called the autophagosome,which subsequently fuses with the vacuole/lysosome. This process similar in mammals. Two sets of genes, APG and AUT, have been identified with this process, and are responsible for two ubiquitin-like systems Apg12 and Apg8, respectively. Apg12 is synthesized in its mature form and seems to have one target, Apg5. Almost all Apg12 molecules are conjugated with Apg5. Aut2/Apg4 processes the Apg8/Aut7 system at its carboxy-terminal region. Apg8 exists in two forms, one is membrane bound through a phospholipid. Lipidation/ activation of Apg8 is mediated by Apg7 and transferred to Apg3 and finally forms a conjugate with phosphatidyl-ethanolamine (PE). Apg4 cleaves Apg8 |
| Immunogen | Recombinant full length protein (S. cerevisiae). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| We recommend the following starting dilutions: ELISA 1:100000, Western Blot 1:4000. Optimal concentration should be determined experimentally by the end user.: |
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| Species Reactivity | Yeast |
| Concentration | 1mg/ml |
| Gene Synonyms | ATG8. FLC3B FLJ25768 GABA(A) receptor-associated protein MM46 Gamma-aminobutyric acid receptor-associated p biosensis. GENE NAME: GABARAP |
|---|---|
| Target | GABARAPL1 |
Application
Western Blot: APG8 Antibody [ - immunization with recombinant yeast APG8 was tested with other anti-UBL antibodies against E.coli lysates expressing the APG8-GFP fusion protein. Panel A shows total protein staining using ponceau. Panel B shows specific reaction with APG8 using a 1:4,000 and 1:8,000 dilution of IgG fraction of Rabbit-anti-APG8 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX . All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4C. E.coli lysate proteins were separated by SDS-PAGE using a 15% gel. Similar experiments where other UBL fusion proteins were separated and probed with this antibody showed no reactivity of anti-APG8 with other UBLs.| Application Notes | We recommend the following starting dilutions: ELISA 1:100000, Western Blot 1:4000. Optimal concentration should be determined experimentally by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.15M Sodium Chloride, 0.02M Potassium Phosphate [pH 7.2] |
