Application
Immunoprecipitation of GABPA protein from 293T whole cell extracts using 5 μg of GABPA antibody [N2C2], Internal . Western blot analysis was performed using GABPA antibody [N2C2], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABPA protein stained by GABPA antibody [N2C2], Internal diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
GABPA antibody detects GABPA protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GABPA antibody diluted at a dilution of 1:1000.| Product Name | GABPA antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GABPA (GA binding protein transcription factor, alpha subunit 60kDa)This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GABPA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (97%), Rat (99%), Xenopus laevis (80%), Chicken (88%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | E4TF1-60 antibody E4TF1A antibody NFT2 antibody NRF2 antibody NRF2A antibody GABPA antibody nuclear respiratory factor 2 alpha subunit antibody GA-binding protein alpha chain antibody GABP subunit alpha antibody transcription factor E4TF1-60 antib |
|---|---|
| Molecular Weight(MW) | 51 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunoprecipitation of GABPA protein from 293T whole cell extracts using 5 μg of GABPA antibody [N2C2], Internal . Western blot analysis was performed using GABPA antibody [N2C2], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABPA protein stained by GABPA antibody [N2C2], Internal diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
GABPA antibody detects GABPA protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GABPA antibody diluted at a dilution of 1:1000.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
