Application
WB analysis of human brain cortex tissue lysate using GAD65 antibody [N-GAD65] followed by Goat anti Mouse IgG H+L:DyLight®800 secondary antibody| Product Name | GAD65 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [N-GAD65] to GAD65This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008] |
| Immunogen | Keyhole Limpet Haemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminal region of human GAD65. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | N-GAD65 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPRIAWB |
| Western Blotting: 1/100 - 1/1000.: |
|
| Species Reactivity | HumanMonkeyRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GAD2 glutamate decarboxylase 2 (pancreatic islets and brain 65kDa) |
|---|---|
| Target | GAD65 |
| Tissue Specificity | This antibody recognizes human glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform or GAD-65. |
Application
WB analysis of human brain cortex tissue lysate using GAD65 antibody [N-GAD65] followed by Goat anti Mouse IgG H+L:DyLight®800 secondary antibody| Application Notes | Western Blotting: 1/100 - 1/1000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide (NaN3) |
