Application
Immunofluorescent staining of 3T3 fibroblasts with Monoclonal Anti-γ-Adaptin (AP-1, Clone 100/3) .| Product Name | gamma Adaptin antibody [100/3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [100 / 3] to gamma AdaptinAdaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. |
| Immunogen | Full length protein (Cow). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 100/3 |
| Host Species | Mouse |
| Tested Applications | EMICC/IFIPWB |
| Electron Microscopy: Use at a dilution of 1/100. IP: Use at a dilution of 1/100. WB: Use at a dilution of 1/100. Detects a band of approximately 104 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Bovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ADTG CLAPG1 |
|---|---|
| Target | gamma Adaptin |
| Tissue Specificity | The antibody reacts with polypeptides of approximately 100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources, or from rat pheochromocytoma cultured cells (PC12), neuroblastoma or astrocytes. This suggests that the 110 and 115 kDa polypeptides may be specific variants that occur only in some cell types of brain. The antibody does not recognize the gamma-subunit in rat and mouse. Using immunofluorescent staining on MDBK cells, and African green monkey kidney cells the binding of the antibody appears to be largely confined to the trans-Golgi network. |
Application
Immunofluorescent staining of 3T3 fibroblasts with Monoclonal Anti-γ-Adaptin (AP-1, Clone 100/3) .| Application Notes | Electron Microscopy: Use at a dilution of 1/100. IP: Use at a dilution of 1/100. WB: Use at a dilution of 1/100. Detects a band of approximately 104 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide |
