Application
Western blotting analysis of gamma-Tubulin in porcine brain lysate by antibody TU-32.| Product Name | gamma tubulin antibody [TU-32] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [TU-32] to gamma tubulinThe gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface. Entrez Gene (human): 17q21The Human Protein Reference Database |
| Immunogen | human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | TU-32 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB: Use at 0.5 µg/mL. Note: excellent | |
| Species Reactivity | HumanMouseChickenRatAmphibiansFungiReptilesPlantBovine |
| Concentration | 1mg/ml |
| Target | gamma-tubulin |
|---|---|
| Tissue Specificity | The antibody TU-32 recognizes an epitope (amino acids 434-449 in human) within C-terminus of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). |
Application
Western blotting analysis of gamma-Tubulin in porcine brain lysate by antibody TU-32.| Application Notes | WB: Use at 0.5 µg/mL. Note: excellent |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
