Application
Western blot analysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate.Application
Confocal immunofluorescence analysis of methanol-fixed HepG2 (left) and Hela (right) cells using anti-GAPDH mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using anti-GAPDH mAb with DAB staining.| Product Name | GAPDH antibody [1A10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1A10] to GAPDHThe product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] |
| Immunogen | The immunogen corresponding to human GAPDH expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1A10 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ELISA: 1/10000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GAPD , G3PD |
|---|---|
| Target | GAPDH |
| Molecular Weight(MW) | 37 kDa (note) |
| Cellular Localization | Postnuclear and Perinuclear regions. |
Application
Western blot analysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate.
Application
Confocal immunofluorescence analysis of methanol-fixed HepG2 (left) and Hela (right) cells using anti-GAPDH mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using anti-GAPDH mAb with DAB staining.| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
