Application
Immunofluorescence: Human neuroblastma line Sh-SY5Y stained with anti-GAPDH [green] and an anti-NF-H [red], counterstained with a fluorescent DNA probe [blue].Application
Application of anti-GAPDH antibody as a protein loading control for Western Blots. Total protein from HeLa, Jurkat and Daudi cell lines were blotted with anti-GAPDH monoclonal antibody [1D4].| Product Name | GAPDH antibody [1D4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse anti-Glyceraldehyde 3-PDH (GAPDH 1D4) monoclonal antibodyGlyceraldehyde 3-phosphate dehydrogenase (GAPDH) is responsible for one of the steps in the glycolytic pathway. GAPDH is essential for cellular metabolism and is thus classified as a ‘house keeping’ protein, relatively high levels of the protein and its RNA being maintained all of the time. GAPDH is highly conserved across species. |
| Immunogen | Mice were immunized with purified pig GAPDH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 1D4 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB: Use at 1/1,000 – 1/5,000 | |
| Species Reactivity | HumanMouseDogPigRatBirdMammalian speciesBovine |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | 396823, GAPD, GAPDH |
|---|---|
| Target | GAPDH |
| Tissue Specificity | reacts with GAPDH from avian, bovine, human, mouse, porcine and rat. Since GAPDH is one of the most conserved proteins known, it is likely that the antibody is effective on other species also. |
| Cellular Localization | Cytoplasmic |
Application
Immunofluorescence: Human neuroblastma line Sh-SY5Y stained with anti-GAPDH [green] and an anti-NF-H [red], counterstained with a fluorescent DNA probe [blue].
Application
Application of anti-GAPDH antibody as a protein loading control for Western Blots. Total protein from HeLa, Jurkat and Daudi cell lines were blotted with anti-GAPDH monoclonal antibody [1D4].| Application Notes | WB: Use at 1/1,000 – 1/5,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20º C. Avoid multiple freeze/thaw cycles. |
| Storage Buffer | 10mM Sodium Azide added as a preservative |
