Application
WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3)Application
1:2000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6)| Product Name | GAPDH antibody [GA1R] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [GA1R] to GAPDHThe product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversibleoxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamideadenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to thislocus are present in the human genome. (provided by RefSeq) |
| Immunogen | Recombinant GAPDH |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GA1R |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCWB |
| WB (with ECL): Use at a dilution of 1:1000-10000 | |
| Species Reactivity | HumanMouseChickenRatHamsterRabbitOtherS.cerevisiaeBacteria |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | glyceraldehyde-3-phosphate dehydrogenase, G3PD, MGC88685, glyceraldehyde3phosphate dehydrogenase, GAPD, GAPDH |
|---|---|
| Target | GAPDH |
| Molecular Weight(MW) | 37 kDa (note) |
| Tissue Specificity | Recognizes native and denatured forms of GAPDH (∼37kDa). Recognizes GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster. GAPDH from other species may also be detectable. |
Application
WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3)
Application
1:2000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6)| Application Notes | WB (with ECL): Use at a dilution of 1:1000-10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods. Avoid repeated freeze-thaw cycles |
| Storage Buffer | 10mM PBS, pH 7.2 |
