Application
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis. A. 30 µg yeast whole cell lysate/extract 10% SDS-PAGE GAPDH antibody [GT239] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg 293T whole cell lysate/extract D. 30 µg A431 whole cell lysate/extract E. 30 µg HeLa whole cell lysate/extract F. 30 µg HepG2 whole cell lysate/extract G. 30 µg H1299 whole cell lysate/extract H. 30 µg HCT116 whole cell lysate/extract I. 30 µg MCF-7 whole cell lysate/extract J. 30 µg NT2D1 whole cell lysate/extract K. 30 µg PC-3 whole cell lysate/extract L. 30 µg U87-MG whole cell lysate/extract 10% SDS-PAGE GAPDH antibody [GT239] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: GAPDH protein stained by GAPDH antibody [GT239] diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis. A. 30 µg whole zebrafish lysate/extract 10% SDS-PAGE GAPDH antibody [GT239] dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
GAPDH antibody [GT239] detects GAPDH protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. GAPDH antibody [GT239] dilution: 1:200.| Product Name | GAPDH antibody [GT239] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT239] to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT239 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
WB:1:500~1:2000 IHC:1:50~1:200 ICC/IF:1:100~1:500 |
|
| Species Reactivity | DrosophilaE.coliHumanMonkeyMouseRatYeastZebra Fish |
| Predicted Reactivity | Xenopus laevis, Dog, Cat, Pig, Rabbit, Chicken, Sheep, Rhesus Monkey, Bovine, Guinea pigXenopus laevis (85%), Dog (95%), Cat (96%), Pig (95%), Rabbit (94%), Chicken (92%), Sheep (94%), Rhesus Monkey (99%), Bovine (94%), Guinea pig (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | G3PD antibody, GAPD antibody, MGC88685 antibody, GAPDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, aging-associated gene 9 protein antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, peptidyl-cysteine S-nitrosylase GAPDH an |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (3) |
| Cellular Localization | Cytoplasm , perinuclear region , Membrane |
Application
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis. A. 30 µg yeast whole cell lysate/extract 10% SDS-PAGE GAPDH antibody [GT239] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg 293T whole cell lysate/extract D. 30 µg A431 whole cell lysate/extract E. 30 µg HeLa whole cell lysate/extract F. 30 µg HepG2 whole cell lysate/extract G. 30 µg H1299 whole cell lysate/extract H. 30 µg HCT116 whole cell lysate/extract I. 30 µg MCF-7 whole cell lysate/extract J. 30 µg NT2D1 whole cell lysate/extract K. 30 µg PC-3 whole cell lysate/extract L. 30 µg U87-MG whole cell lysate/extract 10% SDS-PAGE GAPDH antibody [GT239] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: GAPDH protein stained by GAPDH antibody [GT239] diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis. A. 30 µg whole zebrafish lysate/extract 10% SDS-PAGE GAPDH antibody [GT239] dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
GAPDH antibody [GT239] detects GAPDH protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. GAPDH antibody [GT239] dilution: 1:200.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
WB:1:500~1:2000 IHC:1:50~1:200 ICC/IF:1:100~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
