Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GAPDH antibody at 1:100 dilution.Application
Immunofluorescence analysis of methanol-fixed HeLa, using GAPDH antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE GAPDH antibody diluted at 1:1000Application
GAPDH antibody [N2C1], Internal detects GAPDH protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg BCL-1 whole cell lysate/extract C. 30 µg Raw264.7 whole cell lysate/extract 10 % SDS-PAGE GAPDH antibody [N2C1], Internal dilution: 1:2000| Product Name | GAPDH antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Cat, Pig, Rabbit, Rhesus MonkeyRat (100%), Dog (100%), Cat (100%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | G3PD antibody, GAPD antibody, MGC88685 antibody, GAPDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, aging-associated gene 9 protein antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, peptidyl-cysteine S-nitrosylase GAPDH antibody |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (4) |
| Cellular Localization | Cytoplasm , perinuclear region , Membrane |
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GAPDH antibody at 1:100 dilution.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using GAPDH antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE GAPDH antibody diluted at 1:1000
Application
GAPDH antibody [N2C1], Internal detects GAPDH protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg BCL-1 whole cell lysate/extract C. 30 µg Raw264.7 whole cell lysate/extract 10 % SDS-PAGE GAPDH antibody [N2C1], Internal dilution: 1:2000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
