Application
Anti-GATA4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with GATA4Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA4.| Product Name | GATA4 antibody [1H8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1H8] to GATA4Transcriptional activator. Binds to the consensus sequence 5'-AGATAG-3'. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. |
| Immunogen | Recombinant fragment expressed in E.coli corresponding to amino acids 296-442of human GATA4 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 1H8 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| IHC 1:50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC126629 |
|---|---|
| Target | GATA4 |
| Tissue Specificity | is specific for amino acids 296-442of human GATA4 |
Application
Anti-GATA4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with GATA4
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA4.| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
