Application
Anti-GBE1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with GBE1Application
Flow cytometric analysis of Hela cells, using anti-GBE1 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.| Product Name | GBE1 antibody [4D9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4D9] to GBE1Required for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells. |
| Immunogen | Full length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 4D9 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GBE |
|---|---|
| Target | GBE1 |
| Tissue Specificity | is specific for human GBE1 (NP_000149). |
Application
Anti-GBE1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with GBE1
Application
Flow cytometric analysis of Hela cells, using anti-GBE1 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
