Mouse monoclonal [SPM135] to GCDFP15Gross cystic disease of the breast is benign premenopausal disorder in which cysts are a predominant pathological lesion. These cysts appear to be formed from excessive apocrine cystic secretions. This fluid is composed of several glycoproteins including a unique 15kDa monomer protein, Gross Cystic Disease Fluid Protein-15 (GCDFP15). Cytosolic analysis of normal tissue specimens from all major organs has demonstrated GCDFP15 in apocrine epithelia, lacrimal, ceruminous and Moll's glands and in numerous serous cells of the submandibular, tracheal, bronchial, sublingual and minor salivary glands. GCDFP15 and prostate specific antigen are co-expressed in androgen receptor-positive breast tumours.
Immunogen
Recombinant protein encoding the excreted domain of human GCDFP15.
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
SPM135
Host Species
Mouse
Tested Applications
IHCIHC-P
IHC-P: Use at a dilution of 1/30 for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. GTX15300 demonstrates cytoplasmic staining. Optimal dilutions/concentrations should be determined by the end user.
Human Breast Carcinoma stained with anti-Bnip3L antibody
Application Notes
IHC-P: Use at a dilution of 1/30 for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. GTX15300 demonstrates cytoplasmic staining. Optimal dilutions/concentrations should be determined by the end user.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.