Application
GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis. A. 30 μg Rat-2 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [GT336] dilution: 1:500Application
GEF-H1 antibody [GT336] detects GEF-H1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: GEF-H1 protein stained by GEF-H1 antibody [GT336] diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis. A. 30 μg C8D30 whole cell lysate/extract B. 30 μg BCL-1 whole cell lysate/extract C. 30 μg Raw264.7 whole cell lysate/extract D. 30 μg C2C12 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [GT336] dilution: 1:500Application
GEF-H1 antibody [GT336] detects GEF-H1 protein at cytoskeleton by immunofluorescent analysis. Sample: MDA-MB-231 cells were fixed in methanol for 5 min. Green: GEF-H1 protein stained by GEF-H1 antibody [GT336] diluted at 1:50.Application
GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract 7.5 % SDS-PAGE GEF-H1 antibody [GT336] dilution: 1:500| Product Name | GEF-H1 antibody [GT336] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT336] to GEF-H1 (Rho/Rac guanine nucleotide exchange factor (GEF) 2)Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT336 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Pig, BovineDog (92%), Pig (96%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp547L106 antibody, DKFZp547P1516 antibody, FLJ45440 antibody, GEF antibody, GEF-H1 antibody, GEFH1 antibody, KIAA0651 antibody, LFP40 antibody, P40 antibody, ARHGEF2 antibody, microtubule-regulated Rho-GEF antibody, Rho/Rac guanine nucleotide exchange |
|---|---|
| Cellular Localization | Cytoplasm , Cell junction , tight junction , Golgi apparatus , Spindle |
Application
GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis. A. 30 μg Rat-2 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [GT336] dilution: 1:500
Application
GEF-H1 antibody [GT336] detects GEF-H1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: GEF-H1 protein stained by GEF-H1 antibody [GT336] diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis. A. 30 μg C8D30 whole cell lysate/extract B. 30 μg BCL-1 whole cell lysate/extract C. 30 μg Raw264.7 whole cell lysate/extract D. 30 μg C2C12 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [GT336] dilution: 1:500
Application
GEF-H1 antibody [GT336] detects GEF-H1 protein at cytoskeleton by immunofluorescent analysis. Sample: MDA-MB-231 cells were fixed in methanol for 5 min. Green: GEF-H1 protein stained by GEF-H1 antibody [GT336] diluted at 1:50.
Application
GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract 7.5 % SDS-PAGE GEF-H1 antibody [GT336] dilution: 1:500| Positive Control | 293T , A431 , HeLa , C8D30 , BCL-1 , Raw264.7 , C2C12 , Rat-2 , *3A6 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
