Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Gelsolin antibody detects Gelsolin protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Gelsolin antibody diluted at 1:500.Application
Gelsolin antibody detects Gelsolin protein at secreted on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. Gelsolin antibody dilution: 1:500.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Gelsolin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Gelsolin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Gelsolin (gelsolin)The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Gelsolin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, Sheep, BovineChicken (80%), Sheep (96%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp313L0718 antibody GSN antibody brevin antibody actin-depolymerizing factor antibody gelsolin antibody |
|---|---|
| Molecular Weight(MW) | 86 kDa (note) (11) |
| Cellular Localization | Isoform 2: Cytoplasm , cytoskeleton , Isoform 1: Secreted |
Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Gelsolin antibody detects Gelsolin protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Gelsolin antibody diluted at 1:500.
Application
Gelsolin antibody detects Gelsolin protein at secreted on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. Gelsolin antibody dilution: 1:500.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Gelsolin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | H1299 , HeLa , human plasma , mouse plasma , rat plasma |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
