Application
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term diluted at 1:500.Application
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term diluted at 1:500.Application
Gelsolin antibody detects Gelsolin protein by western blot analysis. Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Gelsolin antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela 7.5% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Gelsolin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | Gelsolin antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Gelsolin (gelsolin)The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Gelsolin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, Sheep, BovineChicken (81%), Sheep (98%), Bovine (98%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp313L0718 antibody GSN antibody brevin antibody actin-depolymerizing factor antibody gelsolin antibody |
|---|---|
| Molecular Weight(MW) | 86 kDa (note) (10) |
| Cellular Localization | Isoform 2: Cytoplasm , cytoskeleton , Isoform 1: Secreted |
Application
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term diluted at 1:500.
Application
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term diluted at 1:500.
Application
Gelsolin antibody detects Gelsolin protein by western blot analysis. Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000.
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Gelsolin antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela 7.5% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Gelsolin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | H1299 , HeLa , human plasma , rat plasma , mouse plasma |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
